What is a Spectrophotometer?
A spectrophotometer, also known as a spectrometer, is a scientific instrument that decomposes complex light into spectral lines. The measurement range generally includes a visible light region with a wavelength range of 380 to 780 nm and an ultraviolet region with a wavelength range of 190 to 1100 nm.
Principle of Spectrophotometer
The spectrophotometer uses a light source that can generate multiple wavelengths, and passes through a series of spectroscopic devices to generate a light source of a specific wavelength. After the light passes through the sample to be tested, part of the light is absorbed, and the absorbance of the sample is calculated, thereby converting into a sample concentration. The absorbance of the sample is proportional to the concentration of the sample.
Spectroscopic Spectroscopy can be Used to:
The composition of the substance is determined by measuring the absorption or emission spectrum of a substance.
The amount of a substance present alone or mixed with other substances is determined by measuring the signal intensity at an appropriate wavelength.
The reaction process is followed by measuring the relationship between the disappearance of a substrate and the amount of product present.
This spectrophotometer adopts a microprogramming technology. It can be used in a material determination, a quantitative analysis in a visible spectrum field. This equipment is a multipurpose analyzer which is necessary for all general labs. It applies to the medical health, clinical examination, biochemistry, petrochemical engineering, environmental protection, quality control's department and teaching lab of universities and colleges, etc.