The Principle of Ultrasonic Cell Crushing Apparatus and Key Points of Its Purchase

The Principle of Ultrasonic Cell Crushing Apparatus and Key Points of Its Purchase

Ultrasonic cell crushing apparatuses convert electric energy into sound energy through transducer. This kind of energy changes into dense bubbles through the liquid medium, and these bubbles burst rapidly, thus playing the role of breaking cells and other substances.

Principle

The principle of ultrasonic cell crushing apparatuses is not very mysterious and complicated. In short, it transforms electric energy into sound energy through transducer, which is transformed into dense bubbles through liquid medium. These bubbles burst rapidly, thus playing a role in breaking up cells and other substances.

Ultrasound is a kind of elastic mechanical wave in material medium. It is a kind of wave form. Therefore, it can be used to detect physiological and pathological information of human body, that is, diagnostic ultrasound. At the same time, it is also an energy form. When a certain dose of ultrasound is transmitted in the organism, through the interaction between them, it can cause changes in the function and structure of the organism, that is, ultrasound biological effect. The main effects of ultrasound on cells are thermal effect, cavitation effect and mechanical effect. Thermal effect: when ultrasound propagates in the medium, the friction hinders the molecular vibration caused by ultrasound, and transforms part of energy into local high heat (42-43 ℃), because the critical lethal temperature of normal tissue is 45.7 ℃. However, the sensitivity of tumor tissue is higher than that of normal tissue, so the metabolism of tumor cells is blocked at this temperature, and the synthesis of DNA, RNA and protein is affected, thus killing the cancer cells while the normal tissue is not affected. Cavitation effect: under ultrasound irradiation, vacuoles are formed in the organism, and mechanical shear pressure and turbulence are produced along with the vibration and violent explosion of vacuoles, resulting in tumor bleeding, tissue disintegration and necrosis. In addition, when the cavitation bubble breaks, it will produce instantaneous high temperature (about 5000 ℃), high pressure (up to 500 × 104pa), which can make the water vapor dissociate and produce OH and H. The redox reaction caused by OH and H can lead to polymer degradation, enzyme deactivation, lipid peroxidation and cell killing. Mechanical effect is the primary effect of ultrasound. In the process of ultrasound propagation, the media particles alternately compress and expand to form a pressure change, causing cell structure damage. The killing effect is closely related to the frequency and intensity of ultrasound.

Key points for purchase

The ultrasonic generator
The signal generator generates a signal of a specific frequency, which is the frequency of the transducer. Generally, the ultrasonic frequency used in the ultrasonic equipment is 20kHz, 25kHz, 28kHz, 33khz, 40KHz, 60KHZ.

Transducer assembly
The transducer assembly is mainly composed of transducer and horn.

Loudspeaker box
It can effectively reduce the noise produced in the process of work, which can keep the laboratory quiet.

The application of ultrasonic cell crushing apparatuses in our country is in the mature stage, but the application is still not popular. The application range of this instrument (equipment) is very wide, which is incomparable with other instruments and equipment. Therefore, the market potential of the instrument (equipment) is great, and manufacturers are increasing. At the same time, it also causes the relative chaos of ultrasonic cleaning industry and market, which can be described as "a mixture of good and bad".


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